Journal: Nature

Article Title: The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma

doi: 10.1038/nature11114

Figure Lengend Snippet: Top 20 candidate genes that cooperate with Kras G12D to promote mPDA in KCTSB13 mice CISs were scored by tumor frequency with the narrowest 15K kernel spatial distribution of insertion sites. Chr: chromosome; N: number of tumors from which the CIS was found; I, total number of insertions of the CIS in the indicated tumors.

Article Snippet: Human USP9x expression was analyzed by quantitative PCR (q-PCR) using TaqMan gene expressiom assays Hs00245009_m1 (Applied Biosystems) on a 7900HT Real-Time PCR system (Applied Biosystems).

Techniques: Mutagenesis

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Matrix metalloproteinase production by COOH-terminal heparin-binding fibronectin fragment in rheumatoid synovial cells.

doi: 10.1097/01.lab.0000056999.08437.b2

Figure Lengend Snippet: Figure 3. Activation of extracellular signal-regulated kinase (ERK), p38 mitogen- activated protein kinase (MAPK), and c-Jun NH2-terminal kinase (JNK) in COOH-HBFN-f–stimulated RSF. After incubation with 100 nM COOH-HBFN-f (indicated as HBFN-f) or 2 ng/ml IL-1 for the time periods indicated, RSF were lysed as described. Cell lysates were subjected to SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-pospho-ERK1/2 (ph-ERK1/2), anti-ERK1/2, anti-phospho-p38 MAPK (ph-p38), anti-p38, anti-phospho-JNK (ph-JNK), or anti-JNK antibody. RSF derived from four patients with RA were used for experiments with similar results.

Article Snippet: Anti-ERK (K-23; sc-94) and -p38 MAPK (C-20; sc-535) were purchased from Santa Cruz Biotechnology, Inc. Anti-phospho-ERK, -phospho-p38 kinase, -JNK, and -phospho-JNK were obtained from Cell Signaling Technology (Beverly, Massachusetts).

Techniques: Activation Assay, Incubation, SDS Page, Derivative Assay

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Matrix metalloproteinase production by COOH-terminal heparin-binding fibronectin fragment in rheumatoid synovial cells.

doi: 10.1097/01.lab.0000056999.08437.b2

Figure Lengend Snippet: Figure 4. Effects of MAPK inhibitors on MMP production by RSF stimulated with COOH-HBFN-f. After preincubation with PD98059 or SB203580 for 1 hour at the concentrations indicated, RSF were incubated for 48 hours with 100 nM COOH-HBFN-f (indicated as HBFN-f). Conditioned media were analyzed by immunoblotting using specific antibodies for MMP-1, MMP-3, and MMP-13. The amount of sample applied was determined on the basis of DNA content of RSF in the well. RSF derived from four patients with RA were used for experiments with similar results.

Article Snippet: Anti-ERK (K-23; sc-94) and -p38 MAPK (C-20; sc-535) were purchased from Santa Cruz Biotechnology, Inc. Anti-phospho-ERK, -phospho-p38 kinase, -JNK, and -phospho-JNK were obtained from Cell Signaling Technology (Beverly, Massachusetts).

Techniques: Incubation, Western Blot, Derivative Assay

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Matrix metalloproteinase production by COOH-terminal heparin-binding fibronectin fragment in rheumatoid synovial cells.

doi: 10.1097/01.lab.0000056999.08437.b2

Figure Lengend Snippet: Figure 7. Activation of ERK, p38, and JNK in RSF with 41 integrin ligation with CS-1. After incubation with CS-1 at 10 M for the time periods indicated, RSF were lysed as described. Cell lysates were subjected to SDS-PAGE, transferred to nitrocellulose, and immunoblotted with anti-ph-ERK1/2, anti-ERK1/2, anti-ph- p38, anti-p38, anti-ph-JNK, or anti-JNK antibody. RSF derived from four patients with RA were used for experiments with similar results.

Article Snippet: Anti-ERK (K-23; sc-94) and -p38 MAPK (C-20; sc-535) were purchased from Santa Cruz Biotechnology, Inc. Anti-phospho-ERK, -phospho-p38 kinase, -JNK, and -phospho-JNK were obtained from Cell Signaling Technology (Beverly, Massachusetts).

Techniques: Activation Assay, Ligation, Incubation, SDS Page, Derivative Assay

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Matrix metalloproteinase production by COOH-terminal heparin-binding fibronectin fragment in rheumatoid synovial cells.

doi: 10.1097/01.lab.0000056999.08437.b2

Figure Lengend Snippet: Figure 8. Effects of MAPK inhibitors on MMP induction by 41 integrin stimulation with CS-1. RSF were pretreated for 1 hour with the indicated concentrations of PD98059 or SB203580 and thereafter stimulated for 48 hours with 10 M CS-1. Conditioned media were analyzed by SDS-PAGE and immunoblotting using specific antibodies for MMP-1, MMP-3, and MMP-13. The amount of sample applied was determined on the basis of DNA content of RSF in the well. RSF derived from four patients with RA were used for experiments with similar results.

Article Snippet: Anti-ERK (K-23; sc-94) and -p38 MAPK (C-20; sc-535) were purchased from Santa Cruz Biotechnology, Inc. Anti-phospho-ERK, -phospho-p38 kinase, -JNK, and -phospho-JNK were obtained from Cell Signaling Technology (Beverly, Massachusetts).

Techniques: SDS Page, Western Blot, Derivative Assay